Troponin I

Cardiac Troponin I (CTnI) is a cardiac muscle protein with a molecular weight of 2.5 kilodaltons. Together with Troponin T (TnT) and Troponin C (TnC), TnI forms a Troponin complex in the heart to play a fundamental role in the transmission of intracellular calcium signal actin-myosin interaction.  The human cTnI  has additional amino acid residues on its N-terminal that do not exist on the skeletal forms thus making cTnI a specific marker for indicating cardiac infarction.  cTnI is released rapidly into blood after the onset of acute myocardial infarction (AMI).   Its release pattern is similar to CK-MB (4 - 6 hours after the onset of AMI).  However, CK-MB level returns to normal after 36 - 48 hours, while levels of cTnI remain elevated for up to 6 - 10 days.  The level of cTnI is very low in normal healthy people, and not detected in patients with skeletal muscle injury.  Therefore, cTnI is a specific marker for diagnosis of AMI.

The Smart Check Troponin I test is an immunochromatography based , one step in vitro test.  It is designed for qualitative determination of cardiac Troponin I (cTnI) in human whole blood specimens as an aid in he diagnosis of myocardial infarction.  Smart Check Troponin I is designed to yield a positive result for TnI concentrations at 1.0 ng/ml or greater.

Smart Check Troponin I test is a sandwich immunoassay.  When sample is added to the sample pad, it moves through the conjugate pad and mobilizes gold anti-cTnI conjugate that is coated on the conjugate pad.  The mixture moves along the membrane by capillary action and reacts with anti-cTnI antibody that is coated on the test region. If cTnI is present at levels of 1.0 ng/ml or greater, the result is the formation of a coloured band in the test region.  If there is no cTnI in the sample, the area will remain colourless.  The sample continues to move to the control area and forms a pink to purple colour, indicating the test is working and the result is valid.

Smart Check Troponin I (Whole Blood)

Metachem Diagnostics Ltd.
29 Forest Road, Piddington, Northampton. NN7 2DA
Tel. (01604) 870370     Fax (01604) 870194