Anti-sera 

Manufactured by B-Bridge International Inc
  

    

Anti-Toa1p antibody

Immunized Animal: Rabbit

Polyclonal antiserum

Cat. # 62-022 (250 μl)

Description

The fundamental transcription factor TFIIA stabilizes the DNA binding of TATA box-binding protein (TBP). It also plays a very important role in the activation of fundamental transcription factor TFIID by the function of the transcription regulator factor. The budding yeast TFIIA is composed of two subunits, Toa1p (286aa) and Toa2p (122aa).

The product is prepared by immunizing rabbit with recombinant protein which was over-expressed in E. coli with a plasmid carrying the entire Toa1p protein (1-286aa) of budding yeast, and purified by chromatography.

Using the product as antiserum in Western blotting, the band of 48 kD pertaining to Toa1p was obtained from the extract of yeast cells (Fig. 1).

Applications

• It can be used in Western blotting or ELISA for the detection and titration of budding yeast Toa1p.

Specifications

Form: 0.1% sodium azide added to the antiserum.
Storage: 4oC

                                                               

Fig. 1: Detection of Toa1p by Western blotting using the Toa1p antibody. 
            Lane 1, extract of budding yeast. 
            The antiserum was diluted 5000 fold before use.

                                                                                                                                 Ver. 092006antitoa1p

For in vitro use only

  

Metachem Diagnostics Ltd. 29 Forest Road, Piddington, Northampton. NN7 2DA.
Tel. (01604) 870370     Fax (01604) 870194