Anti-sera 

Manufactured by B-Bridge International Inc
  

    

Anti-Tfg2p antibody

Immunized Animal: Rabbit

Polyclonal antiserum

Cat. # 62-019 (50 μl)

Description

The basal transcription factor TFIIF binds with RNA polymerase II and takes part in the transcription initiation reaction and the elongation reaction. The budding yeast TFIIF is composed of three subunits, Tfg1p (735 aa), Tfg2p (400 aa) and Taf14p (244 aa).

The product is prepared by immunizing rabbit with recombinant protein which was over-expressed in E. coli with a plasmid carrying the entire region of Tfg2p protein (1-400aa) of budding yeast, and purified by chromatography.

Using this antiserum in Western blotting, the band of 54 kD corresponding to Tfg2p was obtained from the extract of yeast cells (Fig. 1).

Applications

• It can be used in Western blotting or ELISA for the detection and titration of budding yeast Tfg2p.

Specifications

Form: 0.1% sodium azide added to the antiserum.
Storage: 4oC

                                           

Fig. 1: Detection of Tfg2p by Western blotting using the Tfg2p antibody.
              Lane 1, extract of budding yeast. 
              The antiserum was diluted 5000 fold before use.

                                                                                                                                       Ver. 092006antitfg2p

For in vitro use only

  

Metachem Diagnostics Ltd. 29 Forest Road, Piddington, Northampton. NN7 2DA.
Tel. (01604) 870370     Fax (01604) 870194