Sulforhodamine FLICA Assay Kits

FLICA Apoptosis Detection Kits use a novel approach to detect active caspases. The method is based on a Fluorochrome Inhibitor of Caspases (FLICA).  Once inside the cell, the FLICA inhibitor binds covalently to the active caspase.  These inhibitors are cell permeable and non-toxic.  For detection using red fluorescence, a sulforhodamine-labeled fluoromethyl ketone peptide inhibitor of caspases is used.

Jurkat cells, grown in suspension, were incubated with 1mM staurosporine for 3 hours at 37 ⁰C 
to induce apoptosis.  Cells were then labeled with SR-VAD-FMK for 60 minutes at 37 ⁰C.  Slides 
were prepared and samples viewed through a fluorescence microscope using a broad band filter. 

Sulforhodamine FLICA Kits

For Research Use Only

Metachem Diagnostics Ltd.
29 Forest Road, Piddington, Northampton. NN7 2DA
Tel. (01604) 870370     Fax (01604) 870194