BN QuickPickTM  DNA Fragment
For the rapid isolation of DNA Fragments from gel or liquid

    

The QuickPick  DNA Fragment kit provides a fast and simple means of isolating DNA fragments from gel or from liquid solutions.  The kit and PickO gel excisers are suitable for use with standard and low-melting agarose gels in TAE or TBE buffer.  The method does not require  any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation.  DNA purified using the QuickPick DNA Fragment kit is suitable for DNA sizes from 60 bp to 50 kbp.  Purified DNA fragments can be used for downstream applications such as automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription/translation, restriction mapping, restriction enzyme digestion, cloning and labeling.  DNA fragments can be eluted both Elution Buffer or sterile water.  The incubation of elution mixture at elevated temperatures (e.g. 50o C) is preferred for maximising yields.

Principle
DNA fragments in the agarose gel are released using Gel melting/DNA binding Buffer which contains chaotropic salts.  Released DNA is bound to silica-based magnetic particles (MP) in the presence of Gel melting/DNA binding Buffer.  MPs with the bound DNA are captured with PickPen and contaminants are removed by washing with Wash Buffers 1 and 2.  DNA is then eluted from the MPs with Elution Buffer, TE buffer or sterile water.  The procedure starting from prepared sample and ending with purified DNA lasts less than 15 minutes.

Sample Preparation :

Excision of DNA fragments from agarose gels.
The excision of a gel piece using conventional methods i.e. scalpels  and razor blades has been a tedious step in molecular biology resulting in extended UV exposure increasing the risk of damage to DNA samples.  Moreover, the excised gel pieces are non uniform leading to need  weighing of the individual gel slices for determination of the correct reagent use.  To increase the efficiency and safety of the process we recommend the use of PickO gel exciser method for DNA sample gel-extraction (see below), PickO method is based on the use of disposable, elastic silicone rubber tube.  PickOn for the picking of the gel piece containing the desired DNA fragment.  The method is a one-hand process with high efficiency.  Combined with the QuickPick DNA fragment kit it will give an easy and fast process for DNA purification from gels.       

     

Target the DNA Gel Band and press the PickO 
in the agarose gel

Pick up the Gel DNA piece

Take a tube for melting the Gel DNA piece

Press the elastic PickO with your fingers

The Gel DNA band is ready for purification

Purification Protocol:

                                                                                                                                                                      

 

QuickPickTM DNA Fragment

Catalogue Number

No of Prepaations

DNA Fragment

BN 71001

24 preps

DNA Fragment

BN 71011

3 x 96 preps

                                                                                                                             

                                         

QuickPick DNA Fragment Reagents

Catalogue Number

QuickPick DNA Fragment Magnetic Particles

BN 71100

QuickPick DNA Fragment gel melting/DNA binding Buffer 

BN 71300

QuickPick DNA Fragment Wash Buffer 1

BN 71510

QuickPick DNA Fragment Wash Buffer 2

BN 71520

QuickPick DNA Fragment Elution Buffer

BN 71600

 

For details of the PickPenTM magnetic transfer device click here

FOR RESEARCH USE ONLY - NOT FOR USE IN DIAGNOSTIC PROCEDURES

    

Metachem Diagnostics Ltd.
29 Forest Road, Piddington, Northampton. NN7 2DA
Tel. (01604) 870370     Fax (01604) 870194