Carboxyfluorescein FLICA Assay Kits
FLICA
Apoptosis Detection Kits use a novel approach to detect active caspases. The
method is based on a Fluorochrome Inhibitor of Caspases (FLICA). Once
inside the cell, the FLICA inhibitor binds covalently to the active
caspase. These inhibitors are cell permeable and non-toxic. For
detection using green fluorescence, a carboxyfluorescein-labeled fluoromethyl
ketone peptide inhibitor of caspases is used.
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| Photo A, only one cell appears green - it is apoptotic and stained positive for poly caspase activity with FAM-VAD-FMK reagent. The other cell, which is not visible, did not bind to the reagent and therefore not apoptotic. The same cells, photographed at right under a different wavelength for Hoechst stain appear blue. The cell in the top right of photo B (which appears green in photo A) has a very bright nucleus - its DNA is condensing in the cell, a sign it is dying. | |
Carboxyfluorescein FLICA Kits
| Product | Peptide | Size | Catalogue Number |
| FAM Poly Caspase Assay Kit | FAM-VAD-FMK | 25 tests | RX-201-001 |
| FAM Poly Caspase Assay Kit | FAM-VAD-FMK | 100 tests | RX-201-002 |
| FAM Caspase-1 Assay Kit | FAM-YVAD-FMK | 25 tests | RX-201-003 |
| FAM Caspase-1 Assay Kit | FAM-YFVAD-FMK | 100 tests | RX-201-004 |
| FAM Caspase-2 Assay Kit | FAM-VDVAD-FMK | 25 tests | RX-201-005 |
| FAM Caspase-2 Assay Kit | FAM-VDVAD-FMK | 100 tests | RX-201-006 |
| FAM Caspase-3 Assay Kit | FAM-DEVD-FMK | 25 tests | RX-201-007 |
| FAM Caspase-3 Assay Kit | FAM-DEVD-FMK | 100 tests | RX-201-008 |
| FAM Caspase-6 Assay Kit | FAM-VEID-FMK | 25 tests | RX-201-009 |
| FAM Caspase-6 Assay Kit | FAM-VEID-FMK | 100 tests | RX-201-010 |
| FAM Caspase-8 Assay Kit | FAM-LETD-FMK | 25 tests | RX-201-011 |
| FAM Caspase-8 Assay Kit | FAM-LETD-FMK | 100 tests | RX-201-012 |
| FAM Caspase-9 Assay Kit | FAM-LEHD-FMK | 25 tests | RX-201-013 |
| FAM Caspase-9 Assay Kit | FAM-LEHD-FMK | 100 tests | RX-201-014 |
| FAM Caspase-10 Assay Kit | FAM-AEVD-FMK | 25 tests | RX-201-015 |
| FAM Caspase-10 Assay Kit | FAM-AEVD-FMK | 100 tests | RX-201-016 |
| FAM Caspase-13 Assay Kit | FAM-LEED-FMK | 25 tests | RX-201-017 |
| FAM-Caspase-13 Assay Kit | FAM-LEED-FMK | 100 tests | RX-201-018 |
For Research Use Only
Metachem
Diagnostics Ltd.
29 Forest Road, Piddington, Northampton. NN7 2DA
Tel. (01604) 870370
Fax (01604) 870194