BN QuickPickTM Plasmid DNA
For the rapid purification of Plasmid  DNA

    

The QuickPick Plasmid DNA purification kit provides a rapid and simple means of isolating plasmid DNA from bacteria cells.  The method does not require any organic solvents and eliminates the need for centrifugation, vacuum filtration or column separation.  

Principle
The QuickPick Plasmid DNA purification protocol is based on a modified alkaline lysis procedure followed by binding the plasmid DNA to Plasmid MagaZorbTM Magnetic Particles in the presence of Plasmid DNA Binding Buffer.  The plasmid DNA bound to Plasmid DNA MagaZorbTM magnetic particles is captured by PickPenTM and contaminants are removed by washing with Plasmid DNA Wash Buffer.  The plasmid DNA is then eluted from the magnetic particles with the Plasmid DNA Elution Buffer.  The procedure starting from homogenised prepared sample and ending with purified plasmid DNA lasts less than 20 minutes.

BN QuickPickTM Plasmid DNA isolation protocol

Number tubes 1 to 3 and pipette QuickPick Plasmid DNA kit reagents into tubes as follows :
Tube 1 : 125 mL Plasmid DNA Binding Buffer, 5 mL MagaZorbTMMagnetic Particles
Tube 2 : 200 mL Plasmid DNA Wash Buffer
Tube 3 : 40 mL Plasmid DNA Elution Buffer.

 

  1. Transfer the prepared sample supernatant into Tube 1 which contains Plasmid DNA Binding Buffer and Plasmid MagaZorbTM Magnetic Particles.  Incubate at room temperature for 5-10 minutes while mixing continuously.
Bind at room temperature with continuous mixing for 5-10 minutes
  1. Collect the magnetic particles from Tube 1 with the PickPenTM and wash them by releasing them into Tube 2 (Wash Buffer).
  1. Collect the magnetic particles from Tube 2 with the PickPen and release them into Tube 3 (Elution Buffer). Mix at room temperature for 2-10 minutes. 

Elute at room temperature with continuous mixing for 2-10 minutes
  1. Collect the magnetic particles from Tube 3 and discard them.  The eluate now contains the isolated plasmid DNA and is ready for further analysis. 

Collect the magnetic particles and discard them

        

            

Plasmid DNA from E.col XL1-blue culture isolated with
QuickPick Plasmid DNA kit


QuickPickTM  Plasmid DNA

 Catalogue Number No of Preparations
Plasmid DNA BN 52011 48 preps
Plasmid DNA BN52021 96 preps

For details of the PickPenTM magnetic transfer device click here

FOR RESEARCH USE ONLY - NOT FOR USE IN DIAGNOSTIC PROCEDURES

    

Metachem Diagnostics Ltd.
29 Forest Road, Piddington, Northampton. NN7 2DA
Tel. (01604) 870370     Fax (01604) 870194