Human Adiponectin
	Manufactured by B-Bridge International

Obesity and obesity related disorders are reaching alarming proportions in the US and are on the increase in Europe and Asia. A deeper understanding of the molecular and cellular dynamics of such disorders and their subsequent amelioration will have a far-reaching impact on the quality of life of millions of people worldwide.

Adipocytes (fat cells) express a variety of proteins that influence the homeostatic control of glucose and lipid metabolism. Insulin regulates the translocation and secretion of many of these proteins in response to changes in energy balance. The 30 kDa Adipocyte complement-related protein (Acrp30, now known as adiponectin) is one such protein whose secretion from adipocytes is enhanced by insulin stimulation.

It has been suggested that the development of non-insulin dependent (Type II) diabetes may involve dysregulation of adiponectin secretion.  In support of the link between obesity and Type II diabetes it has been shown that decreased expression of adiponectin correlates with insulin resistance and that adiponectin appears to be a potent insulin enhancer linking adipose tissue and whole-body glucose metabolism.

The B-Bridge Human Adiponectin ELISA kit is designed to measure the concentration of human adiponectin from human serum/plasma, human adipocytes or conditioned medium.

The principle of the assay:  Pre-treated samples and serially diluted standard (recombinant human adiponectin) solutions are added to an appropriate number of wells of the microtitre plate and incubated. Adiponectin in the sample is bound by the primary anti-adiponectin monoclonal antibody immobilised in the well (1^st Reaction). After washing, the secondary rabbit anti-adiponectin antibody is added to each well and allowed to incubate (2^nd Reaction). The secondary rabbit anti-adiponectin polyclonal antibody will bind to the adiponectin trapped in the well in the 1^st Reaction.  After washing, a conjugate of horseradish peroxidase and goat anti-rabbit IgG is added to each well and allowed to incubate (3^rd Reaction).  The detection antibody will recognise and bind to the rabbit anti-adiponectin antibody trapped in the well in the 2^nd Reaction.  After washing, the colorimetric substrate for the enzyme is added to all wells and incubated. The colour development is terminated by the addition of a stop solution.  The intensity of the colour is measured at 450nm.  The concentrations of the samples tested are calculated using the absorbance values of the adiponectin standard solutions assayed at he same time.

 Human Adiponectin ELISA Kit

Sensitivity, 23.4 pg/mL Assay Range, 0.375ng/mL to 12ng/mL Time to Answer, 3 hours 30 minutes Sandwich EIA procedure The mouse anti-human adiponectin monoclonal antibody displays no cross reactivity with other species

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